dot blot造句

• Application of pcr - reverse dot blot in genetic diagnosis of - thalassemia
  地中海贫血基因诊断中的应用
• Dot blot mccallum mccallum splaques
  斑点分子杂交法
• Protocol of dot blot and in situ hybridization for white spot virus
  对虾白斑病毒斑点杂交和原位杂交检测操作规程
• Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium
  从培养基中可检测到可溶性抗人绒毛膜促性腺激素的单链抗体。
• [ results ] all the selected clones gave strong signal in dot bloting and elisa
  [结果]对肽库进行三轮筛选后，噬菌体克隆具有良好的富集效果。
• By northern dot blotting , it also showed that the foreign gene could express in the transgenic plants
  Northern斑点杂交表明，外源基因在转基因植株中正常表达。
• The dna and rna dot blotting revealed transcription of the polyprotein gene into mrna in the vero cell
  比较了dna疫苗和两种常用的弱毒苗（ b87 、 d78 ）的安全性和免疫效力。
• To screen the library , defferential screening had been employed . sixty - four clones were randomly picked to perform dot blot
  随机挑出13个克隆进行限制内切酶分析，结果有10个质粒含有300一600bp的插入片段。
• Twentymo transgenic plants were selected by dna dot blot analysis , 15 of them detected hybridization signals and the positive rate was 68 . 2 %
  通过dnadotblot分析，对22个转化株进行筛选，其中15株检测到杂交斑，阳性率为68
• Dot blot and northern blot studies revealed that amphihmgb expression is restricted mainly to ovaries and guts in adult amphioxus while it is highly expression during embryogenesis
  文昌鱼amphihmgb蛋白不含c ?端酸性尾巴，为一碱性蛋白，等电点为9 . 88 。
• [ methods ] two phage - epitope libraries were screened with two anti - vegf neutralizing monoclonal antibodies - jh121 and vg189 , the clones were tested by dot blotting and elisa
  并通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。 elisa分析阳性噬菌体克隆与抗体的亲和力。
• Another detection method , dot blotting , was built also based on 35s promoter and nos terminator . the two genes were labeled by digoxin to done as probe used in dot blotting
  并将35s启动子和nos终止子用地高辛标记后制备成基因探针，建立了转基因检测的分子杂交技术?点杂交。
• Southern blotting indicated that the pa7 fragment was present in p . pseudoalcaligenes genome probably as one copy . dot blotting indicated that the pa4 , pa7 , pas , pa9 were highly homologous
  对pa7片段进行southern杂交鉴定证明该片段来自于类产碱假单胞菌基因组且可能以单拷贝存在。
• Gene silencing , a common phenomenon in phb production through nuclear transformation , had n ' t been observed in the chloroplast transgenic plants analyzed by northern dot blot
  Northern点杂交检测表明与phb合成相关的三个基因均能在转录水平表达，未出现核转化中经常发生的“基因沉默”现象。
• After selecting kanamycin resistant transformants on ms medium , their dna were extracted for pcr and dot blot . in pcr , the 1 . 488kb fragment was amplified , and the result of dot blot was positive
  生长曲线表明，带有克隆片段的转化株在0 . 5mol lnacl的高渗透压培养基中生长良好，而作为对照的缺失株则几乎不能生长。
• The results of our study of last year found that the secretion levels of ley and mmp - 9 of the same morphology were different . by using highly sensitive dot blotting method , the difference can be clearly detected
  我室在近期研究发现不同的胚胎虽然形态上看没有区别，其le ~ y 、 mmp - 9的分泌却不相同，这种差异可以用点杂交的方法检测到。
• Different hybridization bands were detected from different transgenic plants , which indicated that those transgenic plants came from different transformed events . the result of rna dot blot analysis confirmed the transcription of lea3 gene integrated into strawberry genome
  Rnadotblot分析，转化植株检测到了杂交斑，表明lea3基因在草莓细胞内能够进行正常转录。
• Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells . there was expression of the f protein as shown by indirect immunofluorescent assay . the expression began at 48h post - infection and increased thereafter , as indicated by elisa
  将真核表达质粒pcdna3 - f高压电转化dam和phop基因双突变的减毒鼠伤寒沙门氏菌zj111株( zj111 / pcdan3 - f ) ，并直接转染vero细胞，分别提取细胞总dna和总rna ， dig标记探针均可检测到阳性杂交信号。
• Pcr , pcr - southern blot analysis , southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome . the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw , which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
  ( 3 )细胞核载体pb - adw 、 pbg - adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经pcr 、 pcr - southern 、 southern斑点杂交分析证实， adw基因已整合到莴苣基因组中。
• Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
  最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。